單位：  獸醫學院  獸醫學系  

教師姓名：    王孟亮            

職稱：        教授             

Name：  Min-Liang Wong          

E-mail：mlwong@dragon.nchu.edu.tw

TEL：  886-4-2840369             

FAX：  886-4-2862073           

最高學位： 博士(Ph D)        

簡要經歷：

1980-1982： 中華民國陸軍少尉預備軍官

1982-1983： 國立台灣大學理學院生化科學研究所助教

1990-1992： 美國西雅圖華盛頓大學博士後研究(Department of Microbiology,

              University of Washington)

1992-1999： 國立中興大學獸醫系副教授

1999-迄今：國立中興大學獸醫系教授

專長：

生物化學，病毒學

研究領域：

病毒與細胞之互相作用(virus and cell interactions)

主要教授課程(含大學部及研究所)：

獸醫生物化學，分子腫瘤學，高等生物化學

最近參與的研究計劃案：

1.假性狂犬病毒與肌動蛋白關係之研究

2.豬瘟鑑別試劑之研發

著作目錄：

Ａ.期刊論文：

1.  Barber, G. N., M. Wambach, M.-L. Wong, T. E. Dever, A. G. Hinnebusch, and M. Q. Katze.  

    1993. Tanslational regulation by the interferon-induced double stranded-RNA- activated

    68-kDa protein kinase. Proc. Natl. Acad. Sci. USA 90:4621-4625.

2.  Wong, M.-L. 1994. Polarizability and tensor ellipsoid in the Raman effect. Vibrational

    Spectroscopy. 7:197-199.

3.  Wong, M.-L., T.-J. Chang, and C.-Y Wang. 1995. One step filtration method for isolation of      

    herpesviral DNA. Taiwan J. Vet. Med. Anim. Husb. 65:189-191.

4.  Wong, M.-L., S.-T. Kuo, and T.-J. Chang. 1995. Cloning and sequencing of glycoprotein H

     gene of the pseudorabies virus strain TNL. Taiwan J. Vet. Med. Anim. Husb. 65: 317-325.

5.  Wong, M.-L., J.-J. Liu, C. Huang, C.-M. Hsu, and T.-J Chang. 1996. Construction and

     characterization of glycoprotein gE (gl) deletion mutant of pseudorabies virus strain TNL. 

     Taiwan J. Vet. Med. Anim. Husb. 66:235-242.

6.  Wong, M.-L., C.-Y Wang, and T.-J. Chang. 1997. Topoisomerases are involved in the

     replication and gene expression by pseudorabies virus. Taiwan J. Vet. Med. Anim. Husb.

     67:61-68.

7.  Wong, M.-L., T.-Y.Ho, J.-H. Huang, C.-Y Hsiang,. and T.-J. Chang. 1997. Stimulation of type 

    l topoisomerase gene expression by pseudorabies virus. Arch. Virol. 142:2099-2105.

8.  Liu. J.-J., M.-L. Wong, C. Huang, and T.-J Chang. 1997. Serologic Diagnosis of classical

    swine fever virus: use of recombinant antigens (core and E2) in enzyme-linked

    immunosobent assay. Taiwan J. Vet. Med. Anim. Husb. 67:127-134.

9.  Liu. J.-J., M.-L. Wong, and T.-J Chang. 1998. The recombinant nucleocapsid protein of

    classical swine fever virus can act as a transcriptional regulator. Virus Res. 53: 75-80

10.  Wong, M.-L., J.-J. Liu, Y.-S. Chang, and T.-J Chang.1998. Expression of the glycoprotein

      E2 of the classical swine fever virus in Escherichia coli. J. Vet. Med. Sci. 60: 541-544.

11.  Liu. J.-J., M.-L. Wong, P.-F. Chen, and T.-J Chang. 1998. Cloning, expression and sequence

     analysis of the classical swine fever virus nucleocapsid protein. Virus Genes. 16: 225-234.

12.  Wong, M.-L., and C.-H. Chen. 1998. Evidence for the internal location of action in the

     pseudorabies virion. Virus Res. 56: 191-197.

13.  Wong, M.-L., J.-J. Liu, C. Huang, J.-W. Chen, and T.-J Chang. 1998. Molecular cloning and

     nucleotide sequence of 3’-terminal region of classical swine fever virus LPC vaccine

     strain. Virus.Genes, 17: 213-218.

14.  Wong, M.-L., and Y.-R. Yen. 1998. Protein synthesis in pseudorabies virus infected cells:

     decreased expression of PKR, and effects of 2-aminopurine and adenine. Virus Res. 56:

     199-206.

15.  Wong, M.-L., C.-Y. Lin, J.-J. Liu, and T.-J Chang. 1998. Construction and virulence test of

     genetically engineered pseudorabies virus. Taiwan J. Vet. Med. Anim. Husb. 68: 1-9.

16.  Liu. J.-J., M.-L. Wong, and T.-J Chang. 2000. DNA binding properties of the recombinant

     nucleocapsid protein of classical swine fever virus. Taiwan J. Vet. Med. Anim. Husb. 70:

     1-6.

 17.Wong, M.-L.,, Bao-Ying Peng, Jau-Jin Liu and Tien-Jye Chang.  Cloning and Sequencing of Full-

        lengthcDNA of Classical Swine Fever Virus LPC Strain. Virus Genes 23, 187-192, 2001.

  18.Ou CJ, Wong, M.-L., Chang TJ. 2002, A TEF-1-Element is Required for Activation of the

       Promoter of  Pseudorabies Virus Glycoprotein X Gene by IE180. Virus Genes. 25:241-53.

  19.Ou CJ, Wong, M.-L., Huang C, Chang TJ. 2002, Suppression of Promoter Activity of the LAT

       Gene  by IE180 of Pseudorabies Virus. Virus Genes. 25:227-39.

  20.Chang YY, Wong, M.-L., Lin HW, Chang TJ. 2002, Cloning and regulation of the promoter of

       pseudorabies virus (TNL strain) glycoprotein E gene. Virus Genes. 24:235-41.

  21.Wang HC, Wong, M.-L.. 2003, Lytic infection of pseudorabies virus in the presence of spermine,

       spermidine, or DFMO. Virus Res. 94:121-7.

  22.Lin HW, Chang YY, Wong ML, Lin JW, Chang TJ,2004 ,Functional analysis of virion host 

       shutoff protein of pseudorabies virus.Virology.324:412-8.

  23.Chang YY, Lin HW, Wong ML, Chang TJ.2004,Regulation of the vhs gene promoter of

       pseudorabies virus by IE180 and EP0, and the requirement of a Sp1 Site for the promoter

       function. Virus Genes.28(3):247-58.

  24.Wang HC, Lee SH, Chang TJ, Wong ML.2004,Examination of meat components in commercial

       dog and cat feed by using polymerase chain reaction-restriction fragment length polymorphisms

       (PCR-RFLPs) technique. J Vet Med Sci.66(7):855-9.

 25.Kao YC, Wang HC, Chang TJ, Wong ML.2005 Jan,On the TATA box and transcriptional start site

     of gE  gene of pseudorabies virus: a comparison of three methods.J Virol Methods. ;123(1):95-9.

 26.Wang HC, W-D Chen, S-L Lin, J. P-W Chan & M-L Wong. 2005, Phylogenetic analysis of canine

      parvovirus VP2 gene in Taiwan. Virus Genes .31(2):171-174.

 27.Shih-Chieh Chang, Chao-Chin Chang, Tien-Jye Chang, Min-Liang Wong .2005, Prognostic factors

      associated with survival two years after surgery in dogs with malignant mammary tumors: 79

      cases (1998–2002). J. Am. Vet. Med. Assoc. 227:1625-1629.

 28.Hsien-Chi Wang, Yung-Ching Kao, Tien-Jye Chang and Min-Liang Wong 2005 Inhibition of lytic

     infection of pseudorabies virus by arginine depletion Biochem. Biophys. Res. Commun. 334: 631-

     637.